Journal: BMC Cancer

Article Title: Presence of S100A9-positive inflammatory cells in cancer tissues correlates with an early stage cancer and a better prognosis in patients with gastric cancer

doi: 10.1186/1471-2407-12-316

Figure Lengend Snippet: Expression of S100A9 in gastric cancer and adjacent non-cancerous tissues. ( A ) Different expression value of S100A9 in 72 gastric cancer tissues and paired no-cancerous tissues by analyzing data from illumina Sentrix BeadChip cDNA microarray. (B-E) Immunohistochemical staining of S100A9 in gastric cancer tissues ( B ) metastatic lymph nodes ( C ), chronic gastritis ( D ), and adjacent non-cancerous gastric mucosa ( E ). S100A9 localization was revealed as brown or red granulated loci in the cytoplasm of infiltrating inflammatory cells, especially in mononuclear phagocytes and neutrophil granulocytes. (magnification 200×).

Article Snippet: Expression of S100A9, S100A8 and S100A8/A9 were also detected in Cybrdi tissue microarray slides (IC00-01-001, Cybrdi, Xi'an, China) containing chronic gastritis with metaplasia (57 cases) and gastric carcinoma tissues (23 cases).

Techniques: Expressing, Microarray, Immunohistochemical staining, Staining

Journal: BMC Cancer

Article Title: Presence of S100A9-positive inflammatory cells in cancer tissues correlates with an early stage cancer and a better prognosis in patients with gastric cancer

doi: 10.1186/1471-2407-12-316

Figure Lengend Snippet: Immunofluorescence images of S100A9, S100A8 and S100A8/A9 proteins in tissue microarray slides containing gastric cancer tissues (A-J) and chronic gastritis tissues (K-T), and chronic appendicitis tissues with exacerbation (U-Y). S100A9 and S100A8 were detected by monoclonal antibody, prelabeled with the Zenon Alexa Fluor Mouse IgG Labeling Kit (with green and red fluorescence respectively). The nucleus was stained by DAPI. S100A8/A9 heterodimers were detectable using the dimer-specific antibody 27E10 from BMA Biomedicals prelabeled with green fluorescence. The co-localization of S100A9 and S100A8 or S100A8/A9 was showed in merged pictures ( D , I , N , S , X ) and larger merged pictures ( E , J , O , T , Y ). White arrow in 3 T shows co-localization of S100A9 and S100A8/A9 in chronic gastritis. Bar length, 50 μm.

Article Snippet: Expression of S100A9, S100A8 and S100A8/A9 were also detected in Cybrdi tissue microarray slides (IC00-01-001, Cybrdi, Xi'an, China) containing chronic gastritis with metaplasia (57 cases) and gastric carcinoma tissues (23 cases).

Techniques: Immunofluorescence, Microarray, Labeling, Fluorescence, Staining

Journal: Oncotarget

Article Title: Swiprosin-1 stimulates cancer invasion and metastasis by increasing the Rho family of GTPase signaling

doi:

Figure Lengend Snippet: A. Expression of Swiprosin-1 in 12 cancer and 4 normal human cell lines, including Jurkat T cells as a positive control, was determined using western blot (upper). Densitometric quantification results were obtained from three independent experiments (lower). B. Immunohistochemical analysis of Swiprosin-1 expression in tissue microarray containing 30 normal and 29 cancer tissue sections from human cancer patients. Representative tissues with strong Swiprosin-1 expression are shown. C. Human normal (N) and colorectal cancer tissues (T) were immunostained (left) and subjected to western blot (right) with anti-Swiprosin-1 antibody. Ten patients were independently assessed. A typical immunostaining result is presented. D. Human melanoma tissues from patients ( n = 10) were immunostained with anti-Swiprosin-1 antibody. The intensity of positive staining was quantified using Aperio ImageScope software, and T categories classified by the American Joint Committee on Cancer Melanoma Staging.

Article Snippet: Tissue microarray slides of human cancer patients were purchased from Superbiochips Laboratory (Seoul, Korea).

Techniques: Expressing, Positive Control, Western Blot, Immunohistochemical staining, Microarray, Immunostaining, Staining, Software