Journal: Carcinogenesis

Article Title: Muscadine grape skin extract can antagonize Snail-cathepsin L-mediated invasion, migration and osteoclastogenesis in prostate and breast cancer cells

doi: 10.1093/carcin/bgv084

Figure Lengend Snippet: CatL expression increases with tumor progression. (A) Immunohistochemical (IHC) analysis was performed using a 96 core prostate adenocarcinoma tissue microarray. Representive images of CatL in various stages of prostate cancer show that CatL increases with tumor progression. Bar represents 50 µM. (B) Normal/tumor matched invasive ductal carcinoma (IDC) grades 1 and 3, infiltrating carcinoma (IFC) grade 3, adenocarcinoma (grade 3) and metaplastic carcinoma (MPC) grade 3 were analyzed for expression of CatL by western blot analysis. Mature CatL expression was generally higher in tumor as compared to normal tissue. Alpha (α)-tubulin was used as a loading control. Data are representative of at least three independent experiments.

Article Snippet: Immunohistochemistry Examination of the expression and distribution of CatL in human prostate cancer was performed by immunohistochemistry (IHC) using prostate normal and cancer tissue microarray slides obtained from US Biomax (PR956a, Rockville, MD).

Techniques: Expressing, Immunohistochemical staining, Microarray, Western Blot

Journal: BMC Cancer

Article Title: Presence of S100A9-positive inflammatory cells in cancer tissues correlates with an early stage cancer and a better prognosis in patients with gastric cancer

doi: 10.1186/1471-2407-12-316

Figure Lengend Snippet: Expression of S100A9 in gastric cancer and adjacent non-cancerous tissues. ( A ) Different expression value of S100A9 in 72 gastric cancer tissues and paired no-cancerous tissues by analyzing data from illumina Sentrix BeadChip cDNA microarray. (B-E) Immunohistochemical staining of S100A9 in gastric cancer tissues ( B ) metastatic lymph nodes ( C ), chronic gastritis ( D ), and adjacent non-cancerous gastric mucosa ( E ). S100A9 localization was revealed as brown or red granulated loci in the cytoplasm of infiltrating inflammatory cells, especially in mononuclear phagocytes and neutrophil granulocytes. (magnification 200×).

Article Snippet: Expression of S100A9, S100A8 and S100A8/A9 were also detected in Cybrdi tissue microarray slides (IC00-01-001, Cybrdi, Xi'an, China) containing chronic gastritis with metaplasia (57 cases) and gastric carcinoma tissues (23 cases).

Techniques: Expressing, Microarray, Immunohistochemical staining, Staining

Journal: BMC Cancer

Article Title: Presence of S100A9-positive inflammatory cells in cancer tissues correlates with an early stage cancer and a better prognosis in patients with gastric cancer

doi: 10.1186/1471-2407-12-316

Figure Lengend Snippet: Immunofluorescence images of S100A9, S100A8 and S100A8/A9 proteins in tissue microarray slides containing gastric cancer tissues (A-J) and chronic gastritis tissues (K-T), and chronic appendicitis tissues with exacerbation (U-Y). S100A9 and S100A8 were detected by monoclonal antibody, prelabeled with the Zenon Alexa Fluor Mouse IgG Labeling Kit (with green and red fluorescence respectively). The nucleus was stained by DAPI. S100A8/A9 heterodimers were detectable using the dimer-specific antibody 27E10 from BMA Biomedicals prelabeled with green fluorescence. The co-localization of S100A9 and S100A8 or S100A8/A9 was showed in merged pictures ( D , I , N , S , X ) and larger merged pictures ( E , J , O , T , Y ). White arrow in 3 T shows co-localization of S100A9 and S100A8/A9 in chronic gastritis. Bar length, 50 μm.

Article Snippet: Expression of S100A9, S100A8 and S100A8/A9 were also detected in Cybrdi tissue microarray slides (IC00-01-001, Cybrdi, Xi'an, China) containing chronic gastritis with metaplasia (57 cases) and gastric carcinoma tissues (23 cases).

Techniques: Immunofluorescence, Microarray, Labeling, Fluorescence, Staining